منابع مشابه
Positive interference in Radioimmunoassay determination of serum total T3
Introduction: Determination of thyrotropin, total and free thyroxine and triiodothyronine are widely used as diagnostic methods for thyroid function evaluation. There have been numerous reports of interferences in thyroid hormone immunoassays. Herein, a prominent positive interference is reported for a radioimmunoassay kit of total triiodothyronine. Materials and Methods: In this study, a total...
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Radioimmunoassay of serum estrogen (sum of estrone and estradiol-17f3) in 0.5 ml of human serum is described. After dilution of the serum, the estrogens are adsorbed on a Sephadex G-15 column in the presence of dilute HC1 and then eluted with benzene. After evaporation of the benzene eluate, the estrogens are dissolved in bovine serum albumin (5 g/. liter solution, at pH 8.0). An aliquot of thi...
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Purified human spleen ferritin was labelled with 125I. On Sepharose 6-B gel filtration four species of labelled products were separated: a component with a higher molecular weight than ferritin; a component which is eluted in the same volume as unlabelled ferritin; and two labelled compounds with molecular weights lower than ferritin. When these labelled materials were used in a double antibody...
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A column flow-through radioimmune assay for serum myoglobin has been developed. The assay uses antibodies to human or rhesus monkey myoglobin coupled to Sepharose by cyanogen bromide activation and myoglobin labeled by the Chloramine-T method. The labeled myoglobin is stable over two to three half-lives. Serum myoglobin levels were elevated when serum was taken up to 12 hours after myocardial i...
متن کاملRadioimmunoassay of human serum thyrotrophin.
The double antibody radioimmunoassay of serum thyroid-stimulating hormone (TSH) allows measurement of circulating levels of the hormone in most normal subjects. The serum TSH level in normal subjects is 1.6 +/- 0.8muU/ml. Patients with non-toxic goitre and acromegaly have normal TSH levels. Values are always raised in hypothyroid patients (with primary thyroid disease) and are significantly low...
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ژورنال
عنوان ژورنال: RADIOISOTOPES
سال: 1979
ISSN: 1884-4111,0033-8303
DOI: 10.3769/radioisotopes.28.7_437